In the 2-h in vitro gout inflammation model using human blood, mRNA expression of IL-1β, IL-6, and JAK2, as well as protein expression of IL-1β, IL-6, JAK2, STAT1/3, p-JAK2, and p-STAT1/3, were significantly higher in the model group compared to both the blank control group and the PBS-negative control group (all P < 0.05). This evidence concerns the gene JAK2 and gout.