CD8A and neoplasm: Validation of CD8 + T cell depletion by the depletion antibody from the spleens of treated mice is shown in Fig. 5J. While there was no significant protection in anti-tumor response observed in the irradiated (proximal) tumors (Fig. 5K), depletion of CD8 + T cells completely protected non-irradiated (distal) tumors from the anti-tumor effects of the combination therapy (Fig. 5L).