To investigate whether NAP1L1 p.D349E mediates cardiac hypertrophy in vivo, we generated recombinant adeno-associated virus serotype-9 (AAV9) harboring the human NAP1L1 p.D349E gene with the cardiac troponin T (cTNT) promoter (AAV9-cTNT- hNAP1L1 p.D349E-3flag-EGFP) and wild type (AAV9-cTNT- hNAP1L1-3flag-EGFP. The gene discussed is TNNT2; the disease is cardiac hypertrophy.