To determine whether NFE2L3 can also regulate the cell cycle, flow cytometry was used, and the results showed that NFE2L3 knockdown increased the percentage of G0/G1 phase in 786-O cells and A498 cells (Fig. 6i; Supplementary Fig. S3d); that is, downregulation of NFE2L3 can cause G0/G1 cell cycle arrest, which is consistent with the role of NAT10 in ccRCC. The gene discussed is NAT10; the disease is nonpapillary renal cell carcinoma.