TP53 and cervical squamous intraepithelial neoplasia: To determine whether GATA2 loss occurs before or after acquisition of CIN, we performed CIN FISH on our UW1 USC cohort and found no significant difference in CIN between GATA2hi and GATA2lo USCs (Figure 3A), supporting a model whereby GATA2 loss occurs after TP53 inactivation and acquisition of CIN, and therefore represents a more proximal molecular readout of USC invasion and metastatic potential.