Of note, when we stained HIV-GagiGFP-VSV-G-infected U937 cells for Myo9b and observed them at the confocal microscope, we found no co-localization between GagiGFP and Myo9b at 48 h post-infection (Fig. S1, available in the online Supplementary Material), suggesting that there is no direct interaction between Gag and Myo9b and that the increase in viral production could be due to the increase in infection. Here, MYO9B is linked to infection.