To test whether inhibition of RhoGTPases could affect HIV infection in U937 control or Myo9b-silenced cells, we treated these U937 with inhibitors to RhoA, B and C (C3) or to Rac1 (NSC23766) for 4 h before infection with VSV-G-pseudotyped HIV-1. The gene discussed is RAC1; the disease is HIV infectious disease.