As shown in Figure 5, based on Table 3 and compound–target–signal pathway analysis, Western blot validation was performed on caspase-3, CYCS, IL1β, IL6, PPARα, RXRA, TNF, and NF-κB 65 proteins related to NAFLD in the screening pathways. Here, NFKB1 is linked to metabolic dysfunction-associated steatotic liver disease.