ROC curve analysis revealed that serum levels of NEAT1 can effectively differentiate patients with severe oligospermia from healthy individuals, achieving an AUC of 0.6989 (95% CI: 0.6148 to 0.9269, p = 0.01), with a sensitivity of 53% and specificity of 82% at a threshold greater than 0.53-fold (Fig. 7A). Here, NEAT1 is linked to oligospermia.