Because Merlin phosphorylation at Ser518 is known to inactivate its tumor suppressor function,[29] this was reduced upon agrin silencing resulting in its activation coherent with our previous studies (Figure S8A, Supporting Information).[13e] Consequently, agrin knockdown increased LATS1 (pS909) phosphorylation which was reduced by sAgrin treatment (Figure S8A, Supporting Information), further indicating that the activation of core‐Hippo kinase occurs in the absence of agrin. This evidence concerns the gene NF2 and neoplasm.