Several of the ligand-receptor connections listed above were detected between FOXP3high/+ Tregs and cancer cells, as well (e.g., ICOS-ICOSLG, IL12RB1/B2-IL12, ILR1-IL33, CXCR3-CXCL10/CXCL11, CXCR4-CXCL12) (Fig. 7D, E). This evidence concerns the gene ICOS and cancer.