Furthermore, the cycloheximide (CHX) chase experiments (20 μg /ml, 4, 8 and 12 h) demonstrated that NUB1 overexpression shortened the half-life of PCNA, and NUB1 reduction prolonged its half-life by the degradation dynamics assay in HCC cells (Fig. 3C, D). This evidence concerns the gene PCNA and hepatocellular carcinoma.