TRIM22 and infection: To observe the in vitro replication kinetics of the IAV-H1N1 (Fig. 1N and O) and IAV-H3N2 (Fig. 1P and Q), individual viruses were infected at MOI of 0.05 in overexpression of TRIM22 or TRIM22 knockdown A549 cells, and viral genome copies were quantified at various time points during the infection using quantitative PCR (qPCR).