The introduction of GS reversed the attenuated cell viability, cell growth, and colony formation of HDAC6 and USP9X knockdown GBM cells in both glutamine‐replete and ‐depleted conditions (Figure 5G–L and Figure S5F, Supporting Information), indicating that targeting HDAC6 and USP9X suppresses GBM cell growth by modulating GS protein. This evidence concerns the gene HDAC6 and glioblastoma.