To further refine our model, we employed LASSO regression analysis, which selected the following variables: CD4 count, Hb, platelet count (PLT), ALB, VD, lumber spine BMD, current smoking status, diabetes, history of falls, TDF usage, and HIV RNA load (1000-100000 copies/mL) (Supplementary Figure 1). This evidence concerns the gene CD4 and diabetes mellitus.