Furthermore, expression of the senescence markers, including p53, p16, PAI‐1, γ‐H2AX, and IL‐6 in the HF bulge region of the IGF‐1/SIRT1 DTg mice was comparable to that in age‐matched WT or SIRT1 Tg mice, but significantly lower than that observed in IGF‐1 Tg mice (Figure 3c). Here, TP53 is linked to hydrops fetalis.