In line with these results, 3D migration assays, where a tumor spheroid embedded in the extracellular matrix (ECM) invades isotropically in a multidirectional manner, revealed that TRF2 overexpressing spheroids displayed a significant increase of surface invasive area compared to their respective controls (Fig. 3G, Supplementary Fig. 4B). Here, TERF2 is linked to neoplasm.