Both AAV9-shNC and AAV9-shOTUD7B mice showed significant reductions in HNF4α levels after TAC when compared to sham groups Moreover, OTUD7B knockdown mice exhibited a more robust decrease in HNF4α expression following TAC compared to AAV9-shNC mice (Fig. 5E, F). This evidence concerns the gene OTUD7B and persistent truncus arteriosus.