Urinary protein excretion in normal saline treated lupus mice on 21st to 35th, 28th to 35th and 14th to 35th days in anti-dsDNA+, anti-ENA+, and anti-dsDNA+ + anti-ENA+ autoantibodies groups respectively was significantly higher than that of the normal saline treated non PIL control mice. This evidence concerns the gene ENAH and systemic lupus erythematosus.