Image analysis of IHC stained tumor samples for CD73, CD39, PD-L1, and CD8/FoxP3/PanCK in the TME showed that median CD73 expression in the TME overall was higher in the chemotherapy (7.2% CD73-positive tissue) and CRT (6.4%) cohorts compared with the treatment-naïve cohort (2.8%; p < 0.0001 and p < 0.01, respectively) (Fig. 1A). This evidence concerns the gene NT5E and neoplasm.