Thus, we conducted KEGG pathway analysis with 1162 genes that had combined binding of H3K4me3, ERα, and Flag-Brca1, and the data revealed that the five well-known oncogenic pathways, including the cancer, PI3K-AKT, Ras, MAPK, and Rap1 pathways, were among the top 10 enriched pathways (Fig. 4a and Supplementary data 8). This evidence concerns the gene PIK3CB and cancer.