Therefore, we sampled CSF of the 5xFAD (23) transgenic mouse model of Alzheimer’s disease, which expresses human APP and presenilin including five familial AD mutations, and compared its CSF proteome to the one obtained from corresponding wild-type littermates (WT) at 7 months of age followed by validation of key findings using orthogonal methods (ELISA, Simoa) (Fig. 5A). Here, APP is linked to early-onset autosomal dominant Alzheimer disease.