Most prominent alterations were observed in EGFR (auto-phosphorylation Y1197 and 10 biantennary and triantennary fucosyl-sialo glycans at N603), downstream PI3K-Akt pathway (ERBB2-T1240, MET-S990/T992, AKT-S124/S126), and integrin family (sialo-fucosyl glycans), suggesting site-specific alteration between N-glycosylation and phosphorylation interplay in the TKI-resistant L858R-T790M mutant NSCLC cells. Here, ERBB2 is linked to non-small cell lung carcinoma.