Our comparative analyses indicate that both N90- and N322-linked glycans hinder efficient endosomal entry mediated by the S protein of the early SARS-CoV-2 HU-1 strain, while only glycans at N322 inhibit infection by the Omicron BA.5 and XBB.1.5 variants (Fig. 4 and 5). Here, PROS1 is linked to infection.