To preliminarily explore the feasibility of cancer vaccines based on cancer testis antigen in the immunotherapy of sarcomas, we investigate the expression of Cancer/Testis Antigens (CTA) MAGE‐A4, PRAME, MAGE‐A1, KK‐LC‐1, and NY‐ESO‐1 in bone and soft tissue sarcomas, with the aim of assessing their potential for use in sarcoma immunotherapy and determining their expression levels in different subtypes. Here, PRAME is linked to soft tissue sarcoma.