Thus, we aim to investigate the expression of MAGE‐A4, PRAME, MAGE‐A1, Kita‐Kyushu lung cancer antigen‐1 (KK‐LC‐1) and NY‐ESO‐1 in osteosarcoma (OS), liposarcoma (LPS), leiomyosarcoma (LMS), chondrosarcoma (CS) and undifferentiated pleomorphic sarcoma (UPS) by immunohistochemistry and MI chip to provide a basis for novel immunotherapy of sarcomas. This evidence concerns the gene PRAME and osteosarcoma.