To explore the functional roles of the intergenic ALK fusion—LOC388942‐ALK—in NSCLC, spontaneous fusion was accomplished by designing sgRNAs between exons 19 and 20 of ALK as well as between LOC388942 and SLC8A1 both in A549 and H441 cell lines, which was validated by fluorescence and PCR assay (Figure 2A–C). This evidence concerns the gene ALK and non-small cell lung carcinoma.