In contrast, using a large retrospective cohort in this study, we examined the diagnostic performance of twelve serum cytokines between complicated and uncomplicated appendicitis in children, including IL-1, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, IL-17, TNF-α, IFN-α, and IFN-β. Here, IL4 is linked to appendicitis.