Subgroup Cox regression analysis further demonstrated that elevated VPS25 levels were significantly correlated with poorer prognosis specifically in male patients, individuals under 60 years old, and those with advanced T3/T4 stages, lymph node metastasis, clinical stages III/IV, or poorly to moderately differentiated HNSCC (Figure S3C). The gene discussed is VPS25; the disease is head and neck squamous cell carcinoma.