VPS25 and metastatic malignant neoplasm in the lymph nodes: Subgroup Cox regression analysis further demonstrated that elevated VPS25 levels were significantly correlated with poorer prognosis specifically in male patients, individuals under 60 years old, and those with advanced T3/T4 stages, lymph node metastasis, clinical stages III/IV, or poorly to moderately differentiated HNSCC (Figure S3C).