Figure 4c showed a significant increase in Bax expression alongside a slight decrease in Bcl-2 expression with VC 8 mM and a more pronounced decrease with VC 16 mM. ROS generation also led to a reduction in mitochondrial membrane potential, causing cytochrome C release into the cytosol, which further triggered apoptosis. Figure 4d presents a histogram of the data from Figure 4c. Figure 4e-j represents effect of VC in cancer cells MCF7, non-cancerous HEK 293 cells, and non-cancerous CCL 205. Here, BCL2 is linked to cancer.