Considering our observations, global hypomethylation in TNBC tissues seems to be a positive prognostic marker, as our results showed that individuals with the highest levels of global 5-mC and 5-hmC DNA were associated with poorer response to NACT, higher Ki-67 proliferation levels, and higher histological grade in triple-negative breast cancer tissues. Here, MKI67 is linked to triple-negative breast carcinoma.