The normal breast epithelial cell line MCF10A, the ER+ breast cancer cell YS1.2, and two ER− breast cancer cells, pII and MDA-MB-231, were treated with various concentrations of Ang 1–7 or AVE0991 (MAS-R agonist) (0.1, 1, 10 μM) for 72 h, and an image was taken every 24 h during the treatment period. This evidence concerns the gene MAS1L and breast cancer.