In 2014, three studies from China reported that hypermethylation of specific cfDNA regions (TGR5 promoter, IGFBP7 promoter, INK4A promoter) are detected more frequently in HCC cases compared to controls often including CHB patients, whereas the combined use of AFP seemed to improve the diagnostic accuracy of these markers, especially sensitivity [36,37,38]. Here, CDKN2A is linked to hepatocellular carcinoma.