Subsequent in vitro experiments demonstrated that the expression of E-cadherin in BLCA cells with OBSCN expression deficiency was significantly reduced, while the expression of N-cadherin, Vimentin, Snail, and Slug were significantly increased, indicating that OBSCN expression deficiency enhanced the ability of EMT in BLCA cells (Fig. 3H). This evidence concerns the gene VIM and bladder transitional cell carcinoma.