To better understand how the fusion proteins regulate gene expression, we performed Cut&Tag (C&T) in all 4 tRCC cell lines to profile TFE3 fusion protein binding along with H3K27ac ChIP-seq to identify active cis-regulatory elements. Here, TFE3 is linked to renal cell carcinoma associated with Xp11.2 translocations/TFE3 gene fusions.