Unbiased clustering identified distinct myeloid populations, including monocytes expressing Ly6C and Ccr2; monoACT expressing monocyte and activation/maturation markers (MHCII, CD86, and CD274); tumor-associated macrophages (TAMs) expressing high levels of CD64 and F4/80; cDC1s expressing Xcr1 and CD24; plasmacytoid dendritic cells (pDCs) expressing Siglec-H and CD317; maturing DCs expressing high levels of activation/migration markers such as Ccr7 and MHCII; and a distinct DC cluster expressing high levels of CD11c, CD11b, CD172a, and CD301b, resembling DC2s. The gene discussed is CD86; the disease is neoplasm.