An ideal biomarker had features of high sensitivity and specificity, and can be identified by fully automated technology with low production costs.[25]In our study, though novel CD38high monocytes were identified by high cost scRNA‐seq and CyTOF, it was excited to observe that CD38high monocytes were detectable by lower cost flow cytometry, and with an AUC surpassing that of CRP and PCT to discriminate Sepsis group and Surgery group, which suggested its diagnostic values to distinguish sepsis and non‐infectious systemic inflammation. The gene discussed is CRP; the disease is inflammation.