Our aim in this study was to evaluate the levels of Activating Transcription Factor‐6 (ATF‐6), Inositol‐Requiring Enzyme 1 (IRE‐1), and C/EBP‐Homologous Protein (CHOP), which are critical markers of endoplasmic reticulum (ER) stress, in patients with gestational diabetes mellitus (GDM), a condition increasingly recognized for its complex biochemical and molecular underpinnings. The gene discussed is DDIT3; the disease is gestational diabetes.