Our analysis demonstrated statistically significant alterations in the TME, specifically in the proportions of α-smooth muscle actin-positive (α-SMA+) CAFs, CD68+ tumor-associated macrophages (TAMs), CD19+ B lymphocytes, and distinct T lymphocyte subpopulations (CD4+, CD8+, and FOXP3+ regulatory T cells). This evidence concerns the gene ACTA1 and neoplasm.