Participants with NAFLD exhibited elevated levels of liver enzymes, with AST and ALT being higher in the NAFLD group (AST: 22.72 U/L; ALT: 26.46 U/L) compared to non-NAFLD participants (AST: 20.36 U/L; ALT: 18.08 U/L; both p < 0.05). This evidence concerns the gene GPT and metabolic dysfunction-associated steatotic liver disease.