To investigate the molecular mechanisms triggering anxiety-like behaviors, we performed WB detection of mPFC and BLA brain tissue (Figures 6J–M), found that compared to the control group, WB confirmed a significant reduction in CB1R protein expression in the mPFC (p = 0.0022, Figure 6L) and BLA (p = 0.0148, Figure 6M), indicating that prolonged exposure to dual-frequency EMR impacted the CB1R expression of the ECS within the female mice. The gene discussed is CNR1; the disease is Anxiety.