ATL-1 from AMK  • Decreased cell viability• Promoted DNA fragmentation without necrotic effects.• Increased expression levels of cleaved caspase-3, -7, -8, -9, and cleaved PARP• Increased protein expression levels of anti-survival Bcl-2 family proteins and decreased Bcl-2• ATL-1 showed anti-cancer effect through the activation of caspases and pro-apoptotic Bcl-2 family proteins, and the mitochondrial-dependent pathway is involved. This evidence concerns the gene CASP3 and cancer.