AMK extract  • Reduced AGS cell growth• Increased cell cycle sub-G1 phase• Induced apoptosis via ROS generation• Upregulated mitochondrial depolarization and reduced TMRM-positive fluorescence• Increased cleavage of pro-Caspase-9, PARP, and caspase-3• Altered Bax/Bcl-2 ratio and reduced the level of Bcl-2 and Bcl-xL levels• Increased p38 and JNK phosphorylation and reduced the phosphorylation levels of ERK and AKT• Inhibited cell migrationAMK extract induced apoptosis in gastric cancer cell through intrinsic mitochondrial pathway. The gene discussed is MAPK14; the disease is gastric cancer.