LJT extract  • Inhibited protein expression of cleaved caspase-1 and IL-1β• Suppressed the secretion of IL-1β and IL-18• Inhibited NLRP3 inflammasome assembly through lysosomal degradation of NLRP3• Increased autophagosome production by upregulating ATG5 expressionLJT extract alleviated colitis by inhibiting NLRP3 inflammasome, attributed to the regulation of EZH2/ATG5-mediated autophagy. This evidence concerns the gene NLRP3 and colitis.