Both in vitro and in vivo experiments demonstrated that Indirubin, the major bioactive metabolite of Isatidis Folium, effectively mitigates sepsis-induced pathological damage and suppresses the production of pro-inflammatory molecules, like through inhibition of the EGFR/SRC/PI3K and NF-κB/MAPK signaling pathways. The gene discussed is NFKB1; the disease is Sepsis.