Our results also indicate that infection with L-deficient EMCV mutants not only leads to a reduced suppression of Xbp1 mRNA splicing but may also be associated with lower levels of phosphorylated IRE1 compared to cells infected with wild-type EMCV (EMCV_wt) and the EMCV_2A variant (Figure 2, Figure S3, and Figure S4). Here, ERN1 is linked to infection.