In our study applying miRNA and mRNA NGS followed by RT-qPCR in validation processes, a miRNA panel composed of hsa-miR-196a-5p, hsa-miR-21-3p, hsa-miR-10b-3p, hsa-miR-383-5p, and hsa-miR-490-3p and an mRNA panel, including E2F2, HOXD13, VEGFA, CDC45, AURKB, HOXC10, MYBL2, FABP6, PRLHR, NEUROD6, CBLN1, HRH3, HCN1, and RELN, was constructed that could be helpful in the tissue sample-based diagnosis of GBM in the Hungarian population. The gene discussed is NEUROD6; the disease is glioblastoma.