AKT1 and glioblastoma: In addition, after HFD treatment, there was no significant change in PI3K and AKT protein expression in GBM cells, whereas p-PI3K and p-AKT protein expression was significantly downregulated, suggesting that HFD may exert its anti-GBM effect by regulating the PI3K/AKT signaling pathway (Figure 8e,f).