In addition, results from Western blotting indicated that HFD downregulated the expression of N-cadherin, Vimentin, matrix metalloproteinase (MMP)2, and MMP9 proteins, and upregulated the expression of E-cadherin protein in GBM cells compared with the control (DMSO) (Figure 2c,d). This evidence concerns the gene CDH1 and glioblastoma.