In our previous study, we demonstrated that prNK-lysin exerts its anticancer effect on human HCC cells in vitro through membrane disruption [11] and the inhibition of Fascin-1 expression, which regulates the Wnt/β-catenin signaling pathway by inducing β-catenin degradation and subsequently results in the suppression of MMP-2 and MMP-9 expression [12]. This evidence concerns the gene MMP9 and hepatocellular carcinoma.