To investigate the potential molecular mechanisms of Serobioma in reversing the paracellular permeability changes induced by IBS supernatants, qPCR analyses were performed on RNA extracted from Caco-2 cells at the end of the permeability experiments (Figure 5).The co-incubation of Serobioma and AC/IBS supernatants induced an increase in the expression of ZO-1 compared to supernatants alone, although this difference only reached statistical significance for S1 that was co-incubated with IBS-D supernatants (Figure 5A). Here, TJP1 is linked to irritable bowel syndrome.