In the present work, we were the first to compare full transcriptomes of pancreatic cancer cell lines, one of which (PANC-1) originated from primary ductal adenocarcinoma and another (COLO357) isolated from a metastasis of pancreatic ductal adenocarcinoma to lymph node, under the condition of SOX9 knockdown. The gene discussed is SOX9; the disease is pancreatic ductal adenocarcinoma.