GLO1 and Ascites: The model of liver cirrhosis:(1) Male WR treated with CCl4 and phenobarbital for 8 weeks (early cirrhosis without ascites) or 12–14 weeks (advanced cirrhosis with ascites)(2) Male WR treated with CCl4 for 12–14 weeks, and Glo1 inhibitor (ethyl pyruvate—EP) starting from week 8.Primary rat hepatocytes (pHEP), primary hepatic stellate cells (pHSC) and primary liver sinusoidal endothelial cells (pLSEC) isolated from control and cirrhotic WR.Normal hepatic stellate cells (HSZ-B-S1).