The harmful effects elicited by the EVs of SSc patients in HUVECs were confirmed via the quantitative evaluation of EndMT, which was performed by analyzing the expression of CD31, VE-cadherin, vimentin, and N-cadherin in HUVECs in relation to the specific isotypic control (mouse FITC or PE IgG). Here, PECAM1 is linked to systemic sclerosis.