Compared to patients with HCC, iCCAs occurred more frequently in patients with older age (t = 2.25, P = 0.025), normal serum AFP (χ2 = 36.53, P < 0.001) and elevated CA19-9 (χ2 = 26.15, P < 0.001) levels. This evidence concerns the gene AFP and hepatocellular carcinoma.